Chemical hazard prediction and hypothesis testing using quantitative adverse outcome pathways

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Edward J. Perkins
Kalyan Gayen
Jason E. Shoemaker
Philipp Antczak
Lyle Burgoon
Francesco Falciani
Steve Gutsell
Geoff Hodges
Aude Kienzler
Dries Knapen
Mary McBride
Catherine Willett
Francis J. Doyle
Natàlia Garcia-Reyero

Abstract

Current efforts in chemical safety are focused on utilizing human in vitro or alternative animal data in biological pathway context. However, it remains unclear how biological pathways, and toxicology data developed in that context, can be used to quantitatively facilitate decision-making.  The objective of this work is to determine if hypothesis testing using Adverse Outcome Pathways (AOPs) can provide quantitative chemical hazard predictions.  Current methods for predicting hazards of chemicals in a biological pathway context were extensively reviewed, specific case studies examined and computational modeling used to demonstrate quantitative hazard prediction based on an AOP. Since AOPs are chemically agnostic, we propose that AOPs function as hypotheses for how specific chemicals may cause adverse effects via specific pathways. Three broad approaches were identified for testing the hypothesis with AOPs, semi-quantitative weight of evidence, probabilistic, and mechanistic modeling. We then demonstrate how these approaches could be used to test hypotheses using high throughput in vitro data and alternative animal data. Finally, we discuss standards in development and documentation that would facilitate use in a regulatory context. We conclude that quantitative AOPs provide a flexible hypothesis framework for predicting chemical hazards. It accommodates a wide range of approaches that are useful at many stages and build upon one another to become increasingly quantitative.

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[1]
Perkins, E., Gayen, K., Shoemaker, J., Antczak, P., Burgoon, L., Falciani, F., Gutsell, S., Hodges, G., Kienzler, A., Knapen, D., McBride, M., Willett, C., Doyle, F. and Garcia-Reyero, N. 2018. Chemical hazard prediction and hypothesis testing using quantitative adverse outcome pathways. ALTEX - Alternatives to animal experimentation. (Oct. 2018). DOI:https://doi.org/10.14573/altex.1808241.
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