An advanced in vitro model to assess glaucoma onset

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Sergio Claudio Saccà
https://orcid.org/0000-0001-6973-5978
Sara Tirendi
Sonia Scarfì
https://orcid.org/0000-0002-7079-6919
Mario Passalacqua
https://orcid.org/0000-0003-2779-6259
Francesco Oddone
https://orcid.org/0000-0002-2504-0004
Carlo Enrico Traverso
Stefania Vernazza
Anna Maria Bassi
https://orcid.org/0000-0002-4633-4298

Abstract

Glaucoma is the second leading cause of blindness worldwide. Currently glaucoma treatments aim to lower IOP by decreasing aqueous humor production or increasing aqueous humor outflow through pharmacological approaches or trabeculectomy.


However, the lack of effective cure requires new therapeutic strategies. In this regard, we compared the biological responses of a three-dimensional trabecular meshwork (TM) model with or w/o perfusion bioreactor technology to better understand the early molecular changes induced by prolonged oxidative stress condition. Therefore, we obtained standard 3D cultures from the embedment of TM cells in Matrigel and they were cultured both under static and dynamic conditions. In this regard, we demonstrated that the constant medium nutrients supply, conferred by perfusion bioreactor, improved the adaptive behavior to chronic oxidative stress of 3D TM cultures via offsetting pathway activation.

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How to Cite
Saccà, S., Tirendi, S., Scarfì, S., Passalacqua, M., Oddone, F., Traverso, C., Vernazza, S. and Bassi, A. (2020) “An advanced in vitro model to assess glaucoma onset”, ALTEX - Alternatives to animal experimentation. doi: 10.14573/altex.1909262.
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