IgY antibodies as secondary reagent in FACS analysis

Article Sidebar

pdf
Published: Jan 1, 1996

Main Article Content

Undine Hommel, Ingrid Behn, Sunna Hauschildt
[show affiliations]

Abstract

Avian vitellin antibodies (IgY) obtained after immunisation with mouse IgG and mouse IgM in the presence or absence of Freund's complete adjuvant are suited for labelling with FITC or biotin. In FACS analysis the antibodies prove to be excellent tools in detecting mouse monoclonal antibodies of different isotypes. The working dilution (titre of IgY-FITC) is highest when egg preparations of the secondary and tertiary immune response were used. Double staining of two different antigens can be carried out with direct labelled mab (FITC) and indirect labelled mab by IgY-conjugates (biotin). The two antigens can also be detected by double staining of different isotypes (mabs) by hen anti-mouse IgM FITC and hen anti-mouse IgG biotin-streptavidin-phycoerythrin. The staining obtained with IgY is at least as good or even better than that gained with mammalian secondary antibodies.

Article Details

How to Cite
Hommel, U., Behn, I. and Hauschildt, S. (1996) “IgY antibodies as secondary reagent in FACS analysis”, ALTEX - Alternatives to animal experimentation, 13(Supp. 1), pp. 40–44. Available at: https://www.altex.org/index.php/altex/article/view/2060 (Accessed: 19 April 2024).
Issue
Vol. 13 No. Supp. 1 (1996): Egg yolk antibodies, IgY technology
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Articles are distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is appropriately cited (CC-BY). Copyright on any article in ALTEX is retained by the author(s).