[Development of an IgY-based assay for the detection of Ascaris-suum-antigens] [Article in German]

Main Article Content

Anne Schniering, Rüdiger Schade, Theo Hiepe
[show affiliations]


The presented paper describes the development of a coproantigen-test for the detection of ascariosis in pigs using vitelline chicken antibodies. The method used for these investigations was an indirect two-sided-enzyme-immuno-assay (EIA), which was set up as follows: Affinity-purified rabbit antibody was bound to the microtiter-plate as the catching antibody. After the faeces extract to be tested followed the primary antibody, which was a roughly purified avian antibody (IgY). This again could be detected by an enzyme-conjugated antispecies antibody. Sensitivity was high as 5 nanograms antigen per milliliter buffer could be detected. Cross-reactions with antigen of other intestinal parasites occuring in pigs did not become obvious. Cross-reactions with ascarids of other species were demonstrated as expected. The employment of avian antibodies in a two-species EIA promises high sensitivity as unspecific reactions seldom occur because of the phylogenetic difference between birds and mammals.

Article Details

How to Cite
Schniering, A., Schade, R. and Hiepe, T. (1996) “[Development of an IgY-based assay for the detection of Ascaris-suum-antigens] [Article in German]”, ALTEX - Alternatives to animal experimentation, 13(Supp. 1), pp. 62–65. Available at: https://www.altex.org/index.php/altex/article/view/2064 (Accessed: 19 July 2024).

Most read articles by the same author(s)

1 2 > >>