Guidance document on Good Cell and Tissue Culture Practice 2.0 (GCCP 2.0)

Main Article Content

David Pamies
Marcel Leist
Sandra Coecke
Gerard Bowe
David G. Allen
Gerhard Gstraunthaler
Anna Bal-Price
Francesca Pistollato
Rob B. M. de Vries
Helena T. Hogberg
Thomas Hartung
Glyn Stacey

Abstract

Good Cell and Tissue Culture Practice (GCCP) 2.0 is an updated guidance document from GCCP 1.0 (published by ECVAM in 2005), which was developed for practical use in the laboratory to assure the reproducibility of in vitro (cell-based) work. The update in the guidance was essential as cell models have advanced dramatically to more complex culture systems and need more comprehensive quality management to ensure reproducibility and high-quality scientific data. This document describes six main principles to consider when performing cell culture including characterization and maintenance of essential characteristics, quality management, documentation and reporting, safety, education and training, and ethics. The document does not intend to impose detailed procedures but to describe potential quality issues. It is foreseen that the document will require further updates as the science and technologies evolve over time.

Article Details

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Pamies, D., Leist, M., Coecke, S., Bowe, G., Allen, D. G., Gstraunthaler, G., Bal-Price, A., Pistollato, F., de Vries, R. B. M., Hogberg, H. T., Hartung, T. and Stacey, G. (2022) “Guidance document on Good Cell and Tissue Culture Practice 2.0 (GCCP 2.0)”, ALTEX - Alternatives to animal experimentation, 39(1), pp. 30–70. doi: 10.14573/altex.2111011.
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