A three-dimensional model to study human synovial pathology

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Mathijs G. A. Broeren
Claire E. J. Waterborg
Renske Wiegertjes
Rogier M. Thurlings
Marije I. Koenders
Peter L. E. M. van Lent
Peter M. van der Kraan
Fons A. J. van de Loo

Abstract

Therapeutic agents that are used by patients with rheumatic and musculoskeletal diseases were originally developed and tested in animal models, and although retrospective studies show that these models have limited predictive value, their use has continued because of a lack of good in vitro alternatives. In this study, we developed a 3-dimensional synovial membrane model made of either human primary synovial cell suspensions or a mix of primary fibroblast-like synoviocytes and CD14+ mononuclear cells. We analyzed the composition of the mature micromasses by immunohistochemical staining and flow cytometry and showed that the outer surface forms a lining layer consisting of fibroblast-like and macrophage-like cells, reflecting the in vivo naïve synovial membrane. To model the affected synovial membrane in rheu­matoid arthritis (RA), micromasses were exposed to the pro-inflammatory cytokine tumor necrosis factor alpha (TNF-α), which led to increased pro-inflammatory cytokine expression and production, and to hyperplasia of the membrane. To recreate the synovial membrane in osteoarthritis (OA), the micromasses were exposed to transforming growth factor beta (TGF-β), which led to fibrosis-like changes of the membrane, including increased alpha smooth muscle actin (α-SMA) and increased expression of fibrosis-related genes PLOD2 and COL1A1. Interestingly, the macrophages in the micromasses showed phenotypic plasticity, as prolonged TNF-α or TGF-β stimulation strongly reduced the occurrence of CD163+ M2-like macrophages. We show the plasticity of the micromasses as a synovial model for studying RA and OA pathology and propose that the synovial lining micromass system can be a good alternative for drug testing.

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How to Cite
[1]
Broeren, M., Waterborg, C., Wiegertjes, R., Thurlings, R., Koenders, M., van Lent, P., van der Kraan, P. and van de Loo, F. 2018. A three-dimensional model to study human synovial pathology. ALTEX - Alternatives to animal experimentation. 36, 1 (Oct. 2018), 18-28. DOI:https://doi.org/10.14573/altex.1804161.
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