Characterizing sources of variability in zebrafish embryo screening protocols

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Jon T. Hamm
Patricia Ceger
David Allen
Matt Stout
Elizabeth A. Maull
Greg Baker
Amy Zmarowski
Stephanie Padilla
Edward Perkins
Antonio Planchart
Donald Stedman
Tamara Tal
Robert L. Tanguay
David C. Volz
Mitch S. Wilbanks
Nigel J. Walker


There is a need for fast, efficient, and cost-effective hazard identification and characterization of chemical hazards. This need is generating increased interest in the use of zebrafish embryos as both a screening tool and an alternative to mammalian test methods. A Collaborative Workshop on Aquatic Models and 21st Century Toxicology identified the lack of appropriate and consistent testing protocols as a challenge to the broader application of the zebrafish embryo model. The National Toxicology Program established the Systematic Evaluation of the Application of Zebrafish in Toxicology (SEAZIT) initiative to address the lack of consistent testing guidelines and identify sources of variability for zebrafish-based assays. This report summarizes initial SEAZIT information-gathering efforts. Investigators in aca­demic, government, and industry laboratories that routinely use zebrafish embryos for chemical toxicity testing were asked about their husbandry practices and standard protocols. Information was collected about protocol components including zebrafish strains, feed, system water, disease surveillance, embryo exposure conditions, and endpoints. Liter­ature was reviewed to assess issues raised by the investigators. Interviews revealed substantial variability across design parameters, data collected, and analysis procedures. The presence of the chorion and renewal of exposure medium (static versus static-renewal) were identified as design parameters that could potentially influence study outcomes and should be investigated further with studies to determine chemical uptake from treatment solution into embryos. The information gathered in this effort provides a basis for future SEAZIT activities to promote more consistent practices among researchers using zebrafish embryos for toxicity evaluation.

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How to Cite
Hamm, J. T., Ceger, P., Allen, D., Stout, M., Maull, E. A., Baker, G., Zmarowski, A., Padilla, S., Perkins, E., Planchart, A., Stedman, D., Tal, T., Tanguay, R. L., Volz, D. C., Wilbanks, M. S. and Walker, N. J. (2019) “Characterizing sources of variability in zebrafish embryo screening protocols”, ALTEX - Alternatives to animal experimentation, 36(1), pp. 103–120. doi: 10.14573/altex.1804162.

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