Information resources

3R Guide – overview of guidelines, databases, journals, email lists, regulations and policies

3Rs Microsite

3Rs Reduction – course on experimental design

3Rs Resources - library of 3Rs resources compiled by NC3Rs

ALTBIB – bibliography on alternatives

AltTox – online discussion and information exchange on non-animal testing methods

Animal Ethics Infolink

Animal Welfare, Ethics and the 3Rs – training materials

Animal Welfare Bodies and National Committees

Animalearn - loans alternatives for education

AOP Knowledge Base

AOP-Wiki - repository ofinformation for AOP development effort

ARRIVE guidelines

ATLA - Alternatives to Laboratory Animals – scientific journal

Bioethics Information Center (in Korean)

Charité 3R Toolbox

Costs and Benefits of Animal Experiments

DB-ALM – EURL-ECVAM DataBase Service on ALternative Methods to animal experimentation

DC 3Rs - Inventory of 3Rs knowledge sources

Design of Animal Experiments - Reducing the use of animals in research through better experimental design

EC Regulation on cosmetic products (1223/2009)

EPA Strategic Vision for Adopting New Approach Methodologies for pesticide testing

Ethical principles and guidelines for experiments on animals

EU Directive on the protection of animals used for scientific purposes (2010/63)

EU site on animals used for scientific purposes

EUROECOTOX – alternative and standard assays in ecotoxicological testing

Experimental Design Assistant

Fetal Calf Serum-Free Database

FLAIRE Learning - Flecknell laboratory animal interactive resources for education

Gold Standard Publication Checklist

Good search practice on animal alternatives

Guide for the Care and Use of Laboratory Animals, 8th edition

Guiding Principles on Good Practice for Animal Welfare and Ethical Review Bodies

Hastings Center – educational research on ethics of medical research with animals

Humane Endpoints

Humane Science Course – free online course in humane science and the 3Rs


InterNICHE - International Network for Humane Education

InVitro+Jobs – internet platform for animal-free research

Journal of Applied Animal Welfare Science – scientific journal

Lay Members Handbook – for lay members of ethical review and similar bodies worldwide

Literature Search for Alternatives Worksheet

Montréal Declaration on the Synthesis of Evidence to Advance the 3Rs Principles in Science

NC3Rs Gateway - open access publication of NC3Rs-funded research

NC3Rs Resource hubs

NICEATM Resources for Test Method Developers

NORINA - Norwegian Inventory of Alternatives – audiovisual alternatives to the use of animals in teaching

OECD Adverse Outcome Pathways resources

PARERE Network

PREPARE guidelines

Principles of Humane Experimental Technique

Project Evaluation and Retrospective Assessment

Systematic Review Facility

Toxicity Testing in the 21st Century: A Vision and a Strategy

Toxicology 21: Scientific Applications - course

Toxicology in Vitro – scientific journal

Translational Training Tools – tools for teaching non-surgical and surgical procedures

Virtual pharmacology lab – repository of simulations


Animal Welfare and 3Rs Organizations


3R Center Tübingen for In vitro Models and Alternatives to Animal Testing (Germany)

3Rs Center Czech Republic (Czech Republic)

3Rs-Centre ULS (The Netherlands)

AfR - Animalfree Research (Switzerland)

Alternativfondet (Denmark)

Altertox Academy (Belgium)

AnimalFree Research UK (UK)

Ärzte gegen Tierversuche (Germany)

Ärztinnen und Ärzte für Tierschutz in der Medizin (Switzerland)

Akademie für Tierschutz (Germany)

ALEXANDRA Association (Monaco)

BB3R - Freie Universität Berlin (Germany)

Belgian Platform for Alternative Methods to Animal Testing (Belgium)

Biographics Laboratory 3R (Switzerland)

Bf3R - Deutsches Zentrum zum Schutz von Versuchstieren (Germany)

CAAT-Europe at the University of Konstanz (Germany)

Centro 3R - Interuniversitary Center for the Promotion of the Principles of the 3Rs in Teaching and Research (Italy)

Charité 3R - Charité – Universitätsmedizin Berlin (Germany)

CMCiB-IGTP - Comparative Medicine and Bioimage Centre of Catalonia (Spain)

Comparative Medicine, Trinity College Dublin (Ireland)

Crueltyfree International (UK)

Danish 3R-Center (Denmark)

Department of Animals in Science and Society (The Netherlands)

Doerenkamp-Zbinden Foundation (Switzerland)

Eurogroup for animals (Europe)

European Coalition to End Animal Experiments (ECEAE) (Europe)

ecopa - European consensus-platform for alternatives (Europe)

EPAA - European partnership for alternative approaches to animal testing (Europe)

Erna-Graff-Stiftung für Tierschutz (Germany)

ESTIV - European Society of Toxicology in Vitro (Europe)

EURL-ECVAM - European Union Reference Laboratory for alternatives to animal testing (Europe)

EUROoCs - European Organ-on-Chip Society (Europe)

EUSAAT - European Society for Alternatives to Animal Testing (Austria)

EUToxRisk (Europe)

FICAM - Finnish Centre for Alternative Methods (Finland)

Fincopa - Finnish Consensus Platform for Alternatives (Finland)

Fondation Egon Naef pour la recherche in vitro (Switzerland)

Forska Utan Djurförsök - The Swedish Fund for Research Without Animal Experiments (Sweden)

FRAME - Fund for the Replacement of Animals in Medical Experiments (UK)

Francopa (France)

Iaapea - International Association Against Painful Experiments on Animals (UK)

In vitro Toxicology and Biomedicine, Dept inaugurated by the Doerenkamp-Zbinden Foundation (Germany)

IC-3Rs - Innovation Centre - 3R Alternatives (Belgium)

ICAR3R - Interdisciplinary Centre for 3Rs in Animal Research (Germany)

IPAM (Italy)

IVTIP - In Vitro Testing Industrial Platform (The Netherlands)

LDF - Lord Dowding Fund for humane research (UK)

Leibniz Alternatives at IUF - Leibniz Research Institute for Environmental Medicine (Germany)

Ligue Suisse contre la vivisection (Switzerland)

LIST - Luxembourg Institute of Science and Technology  (Luxembourg)

Menschen für Tierechte – Bundesverband für Tierversuchsgegner e.V. (Germany)

MUI animalFree Research Cluster (Austria)

NC3Rs - National Centre for the Replacement, Refinement and Reduction of Animals in Research (UK)

NCad - Netherlands National Committee for the protection of animals used for scientific purposes (The Netherlands)

Norecopa (Norway)

PETA International Science Consortium (UK)

R2N - "Replace" and "Reduce" based in Lower Saxony (Germany)

REMA (Spain)

RepRefRed Society (Austria)

ROCAM – Romanian Center for Alternative Test Methods (Romania)

RSPCA - Royal Society for the Prevention of Cruelty to Animals (UK)

Schweizer Tierschutz (Switzerland)

set - Stiftung zur Förderung von Ersatz- und Ergänzungsmethoden zur Einschränkung von Tierversuchen (Germany)

Slovak National Platform for 3Rs in Research, Development and Education (Slovakia)

Stiftung für das Tier im Recht (Switzerland)

Stigting Proefdierfrij (The Netherlands)

Swecopa (Sweden)

Swedish 3Rs Center (Sweden)

Swiss 3RCC - 3R Competence Centre (Switzerland)

TARCforce 3R - Medical University Mainz (Germany)

UFAW The Universities Federation for Animal Welfare (UK)

Unit Ethics and Human-Animal Studies, Messerli Research Institute (Austria)

Vier Pfoten International (Austria)

ZEBET: Zentralstelle zur Erfassung und Bewertung von Ersatz- und Ergänzungsmethoden zu Tierversuchen (Germany)

Zürcher Tierschutz (Switzerland)



AFAAR - American Fund for Alternatives to Animal Research (USA)

ARDF - Alternatives Research and Development Foundation (USA)

ASCCT - American Society for Cellular and Computational Toxicology (USA)

AWIC - USDA Animal Welfare Information Center (USA)

CAAT - Center for Alternatives to Animal Testing (USA)

CCAAM - Canadian Centre for Alternatives to Animal Methods (Canada)

CCAC - Canadian Council on Animal Care (Canada)

CSS - Center for Contemporary Sciences (USA)

Doerenkamp-Zbinden endowed chair in evidence-based toxicology (USA)

EBTOX - Evidence-Based Toxicology (EBT) Collaboration (USA)

HIS - Humane Society International (USA)

HSUS - Humane Society of the United States (USA)

ICCVAM - The Interagency Coordinating Committee on the Validation of Alternative Methods (USA)

IFER - International Foundation for Ethical Research (USA)

IIVS - Institute for In Vitro Sciences (USA)

Instituto 1R (Brazil)

NICEATM - NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (USA)

North American 3Rs Collaborative (USA and Canada)

Society for the Advancement of Adverse Outcome Pathways (USA)


Asia and Oceania

ANZCCART- Australian and New Zealand Council for the Care of Animals in Research and Teaching (Australia and New Zealand)

Humane Research Australia (Australia)

JaCVAM - Japanese Center for the Validation of Alternative Methods (Japan)

JSAAE - Japanese Society for Alternatives to Animal Experimentation (Japan)

Konkuk Institute for the Three Rs (Korea)

MAWA Trust - Medical Advances Without Animals (Australia)

NCAAE - National Centre for Alternatives to Animal Experiments (India)

SAAE-India - Society for Alternatives to Animal Experiments (India)

Society of Alternatives to Animal Experiments (Korea)


To suggest further 3Rs relevant links, please contact:

Last update January 4, 2021