Effectivity of different methods for the extraction and purification of IgY

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Published: Jan 1, 1996

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Christine Schwarzkopf, Bernhard Thiele
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Abstract

Extraction of IgY from the egg yolk of immunised laying hens differs from the conventional way of antiserum preparation following blood sampling or exsanguination also with regard to the preparation technique. Six methods of IgY preparation were studied comparatively. As a result, the ammonium sulfate precipitation proved to be the most environmentally compatible and economical one. The advantage of this method becomes particularly obvious when the method is followed by affinity chromatography.

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How to Cite
Schwarzkopf, C. and Thiele, B. (1996) “Effectivity of different methods for the extraction and purification of IgY”, ALTEX - Alternatives to animal experimentation, 13(Supp. 1), pp. 35–39. Available at: https://www.altex.org/index.php/altex/article/view/2059 (Accessed: 17 April 2025).
Issue
Vol. 13 No. Supp. 1 (1996): Egg yolk antibodies, IgY technology
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Articles
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