Identifying, naming and documenting of test and tool compound stocks

Main Article Content

Marcel Leist

Abstract

Handling of chemicals is an often-neglected area of test descriptions. Some important aspects are highlighted here, using methyl-phenyl-tetrahydropyridine (MPTP), ferrous sulfate (FeSO4·xH2O) and ciguatoxin as example compounds. These are used to provide some background on aspects of acid-base equilibria, redox state, crystal water, natural compound mixtures, and chemical naming systems. Also, solvents and impurities are addressed, for instance concerning their often high (millimolar range) concentrations in assay buffers and cell culture media. The discussion of these aspects calls for a more standardized preparation of test solutions and a more extensive disclosure of the procedure in publications; it also suggests more flexibility in data mining, as compounds with clearly different identifiers may have been used to produce highly similar or fully identical test conditions. While this short overview is not intended as definitive guidance, it does demand more active involvement of all test developers and performers with these issues, and it calls for more transparent information disclosure concerning the preparation and use of test and control chemical solutions.

Article Details

How to Cite
Leist, M. (2021) “Identifying, naming and documenting of test and tool compound stocks”, ALTEX - Alternatives to animal experimentation, 38(1), pp. 177-182. doi: 10.14573/altex.2012311.
Section
BenchMarks
References

Bal-Price, A., Hogberg, H. T., Crofton, K. M. et al. (2018). Recommendation on test readiness criteria for new approach methods in toxicology: Exemplified for developmental neurotoxicity. ALTEX 35, 306-352. doi:10.14573/altex.1712081

Coecke, S., Balls, M., Bowe, G. et al. (2005). Guidance on good cell culture practice. A report of the second ECVAM task force on good cell culture practice. Altern Lab Anim 33, 261-287. doi:10.1177/026119290503300313

Daneshian, M., Botana, L. M., Dechraoui Bottein, M.-Y. et al. (2013). A roadmap for hazard monitoring and risk assessment of marine biotoxins on the basis of chemical and biological test systems. ALTEX 30, 487-545. doi:10.14573/altex.2013.4.487

Delp, J., Gutbier, S., Klima, S. et al. (2018). A high-throughput approach to identify specific neurotoxicants / developmental toxicants in human neuronal cell function assays. ALTEX 35, 235-253. doi:10.14573/altex.1712182

Delp, J., Funke, M., Rudolf, F. et al. (2019). Development of a neurotoxicity assay that is tuned to detect mitochondrial toxicants. Arch Toxicol 93, 1585-1608. doi:10.1007/s00204-019-02473-y

Gutbier, S., Spreng, A.-S., Delp, J. et al. (2018). Prevention of neuronal apoptosis by astrocytes through thiol-mediated stress response modulation and accelerated recovery from proteotoxic stress. Cell Death Differ 25, 2101-2117. doi:10.1038/s41418-018-0229-x

Hartung, T., De Vries, R., Hoffmann, S. et al. (2019). Toward good in vitro reporting standards. ALTEX 36, 3-17. doi:10.14573/altex.1812191

Kisitu, J., Bennekou, S. H and Leist, M. (2019). Chemical concentrations in cell culture compartments (C5) – Concentration definitions. ALTEX 36, 154-160. doi:10.14573/altex.1901031

Kisitu, J., Hollert, H., Fisher, C. et al. (2020). Chemical concentrations in cell culture compartments (C5) – Free concentrations. ALTEX 37, 693-708. doi:10.14573/altex.2008251

Krebs, A., Waldmann, T., Wilks, M. F. et al. (2019). Template for the description of cell-based toxicological test methods to allow evaluation and regulatory use of the data. ALTEX 36, 682-699. doi:10.14573/altex.1909271

Krebs, A., van Vugt-Lussenburg, B. A., Waldmann, T. et al. (2020). The EU-ToxRisk method documentation, data processing and chemical testing pipeline for the regulatory use of new approach methods. Arch Toxicol 94, 2435-2461. doi:10.1007/s00204-020-02802-6

Leist, M., Efremova, L. and Karreman, C. (2010). Food for thought … Considerations and guidelines for basic test method descriptions in toxicology. ALTEX 27, 309-317. doi:10.14573/altex.2010.4.309

Leist, M. and Hengstler, J. G. (2018). Essential components of methods papers. ALTEX 35, 429-432. doi:10.14573/altex.1807031

Lotharius, J., Falsig, J., van Beek, J. et al. (2005). Progressive degeneration of human mesencephalic neuron-derived cells triggered by dopamine-dependent oxidative stress is dependent on the mixed-lineage kinase pathway. J Neurosci 25, 6329-6342. doi:10.1523/JNEUROSCI.1746-05.2005

Masjosthusmann, S., Blum, J., Bartmann, K. et al. (2020). Establishment of an a priori protocol for the implementation and interpretation of an in‐vitro testing battery for the assessment of developmental neurotoxicity. EFSA Supporting Publications 17, 1938E. doi:10.2903/sp.efsa.2020.EN-1938

Moné, M. J., Pallocca, G., Escher, S. E. et al. (2020). Setting the stage for next-generation risk assessment with non-animal approaches: The EU-ToxRisk project experience. Arch Toxicol 94, 3581-3592. doi:10.1007/s00204-020-02866-4

Nyffeler, J., Dolde, X., Krebs, A. et al. (2017a). Combination of multiple neural crest migration assays to identify environmental toxicants from a proof-of-concept chemical library. Arch Toxicol 91, 3613-3632. doi:10.1007/s00204-017-1977

Nyffeler, J., Karreman, C., Leisner, H. et al. (2017b). Design of a high-throughput human neural crest cell migration assay to indicate potential developmental toxicants. ALTEX 34, 75-94. doi:10.14573/altex.1605031

OECD (2018) Guidance Document on Good In Vitro Method Practices (GIVIMP). OECD Series on Testing and Assessment, No. 286. OECD Publishing, Paris. doi:10.1787/20777876

Pamies, D., Bal-Price, A., Simeonov, A. et al. (2017). Good cell culture practice for stem cells and stem-cell-derived models. ALTEX 34, 95-132. doi:10.14573/altex.1607121

Pamies, D., Bal-Price, A., Chesné, C. et al. (2018). Advanced good cell culture practice for human primary, stem cell-derived and organoid models as well as microphysiological systems. ALTEX 35, 353-378. doi:10.14573/altex.1710081

Schildknecht, S., Pape, R., Meiser, J. et al. (2015). Preferential extracellular generation of the active parkinsonian toxin MPP+ by transporter-independent export of the intermediate MPDP+. Antioxid Redox Signal 23, 1001-1016. doi:10.1089/ars.2015.6297

Schmidt, B. Z., Lehmann, M., Gutbier, S. et al. (2017). In vitro acute and developmental neurotoxicity screening: An overview of cellular platforms and high-throughput technical possibilities. Arch Toxicol 91, 1-33. doi:10.1007/s00204-016-1805-9

Scholz, D., Chernyshova, Y., Ückert, A.-K. et al. (2018). Reduced Aβ secretion by human neurons under conditions of strongly increased BACE activity. J Neurochem 147, 256-274. doi:10.1111/jnc.14467

Most read articles by the same author(s)

1 2 3 4 5 6 7 > >>